BMG多功能酶标仪国内用户出版物快报 (2023年3月)
本期研究亮点推介
>>>TECHNICAL ADVANCE: Investigating the dynamics of protein-protein interactions in plants
南京林业大学生物与环境学院华南林业可持续发展协同创新中心暨国家林业和草原局亚热带森林生物多样性保护重点实验室,西北农林科技大学草地农学院近日在专业学术期刊The Plant Journal发表了学术研究成果:TECHNICAL ADVANCE: Investigating the dynamics of protein-protein interactions in plants,开发了几种方法来监测植物中蛋白质-蛋白质的相互作用。动态蛋白质复合物的检测是非常具有挑战性的,几乎没有可用于此目的的技术。他们开发了一个新的平台,检测植物中的蛋白质相互作用,通过双分子荧光互补(BiFC)、生物发光共振能量转移和共免疫沉淀分析证实了这些相互作用。通过Förster共振能量转移(FRET)和萤光素酶监测这些相互作用的动力学,同时通过BiFC FRET监测三元蛋白质复合物的结合。研究人员选用了德国的BMG Labtech CLARIOstar Plus来进行蛋白相互作用的检测。BMG Labtech CLARIOstar Plus多功能酶标仪专利的LVF荧光光栅具备可与滤光片系统相媲美的检测灵敏度,并具有荧光光栅的灵活性,为蛋白相互作用研究提供灵活、高灵敏及稳定的探索研究平台。
(原文:https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.16182)
研究领域
(共计27篇,涉及17个领域)
Human serum-derived exosomes modulate macrophage inflammation to promote VCAM1-mediated angiogenesis and bone regeneration
广州医科大学附属口腔医院小儿牙内科
Composites of Silk Nanofibrils and Metal–Organic Framework Nanosheets for Fluorescence-Based Sensing and UV Shielding
南京林业大学化学工程学院江苏省森林资源高效加工与利用协同创新中心
Relationship between systemic inflammation and lung injury induced by chromate exposure: A cross–sectional study in workers
北京大学公共卫生学院职业与环境卫生科学系
Purification, characterization and inactivation kinetics of polyphenol oxidase extracted from Cistanche deserticola
河北科技大学食品科学与生物学院
Structural and mechanistic insights into fungal β-1,3-glucan synthase FKS1
华中科技大学同济医学院基础医学院生物化学与分子生物学教研室
Tectorigenin targets PKACα to promote GLUT4 expression in skeletal muscle and improve insulin resistance in vitro and in vivo
东北师范大学可用药基因与蛋白质筛选国家工程实验室
Network pharmacology associated anti-influenza mechanism research of Qingjie-Tuire Granule via STAT1/3 signaling pathway
广州医科大学第一附属医院
Rutaecarpine attenuates high glucose-induced damage in AC16 cardiomyocytes by suppressing the MAPK pathway
河南大学附属南市医院内分泌科
Sulforaphane Attenuates Glycoprotein VI-mediated Platelet Mitochondrial Dysfunction through Up-regulating cAMP/PKA Signaling Pathway in vitro and in vivo
清华大学化学系有机光电子与分子工程教育部重点实验室
Secreted protease PRSS35 suppresses hepatocellular carcinoma by disabling CXCL2-mediated neutrophil extracellular traps
中国科学技术大学基础医学院
Neuron stem cell NLRP6 sustains hippocampal neurogenesis to resist stress-induced depression
南京大学生命科学学院
TECHNICAL ADVANCE: Investigating the dynamics of protein-protein interactions in plants
南京林业大学生物与环境学院华南林业可持续发展协同创新中心暨国家林业和草原局亚热带森林生物多样性保护重点实验室
Bacteria-Driven Tumor Microenvironment-Sensitive Nanoparticles Targeting Hypoxic Regions Enhances the Chemotherapy Outcome of Lung Cancer
西南医科大学附属医院肿瘤科
Discovery of BGB-8035, a Highly Selective Covalent Inhibitor of Bruton's Tyrosine Kinase for B-Cell Malignancies and Autoimmune Diseases
百济神州(北京)有限公司药物化学部
Quantitative trait loci for rolled leaf in a wheat EMS mutant from Jagger
中国农业大学
α-Lipoic acid loaded hollow gold nanoparticles designed for osteoporosis treatment: preparation, characterization and in vitro evaluation
海军医科大学第二附属医院脊柱外科
Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models
吉林大学第一医院耳鼻咽喉头颈外科
DNA damage and up-regulation of PARP-1 induced by columbin in vitro and in vivo
贵州医科大学药用植物功能与应用国家重点实验室贵州省药物制剂重点实验室
PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma
南开大学医学院病理科
PEG-conjugated bovine haemoglobin enhances efficiency of chemotherapeutic agent doxorubicin with alleviating DOX-induced splenocardiac toxicity in the breast cancer
北京卫生服务与输血医学研究所
Metformin ameliorates calcium oxalate crystallization and stone formation by activating the Nrf2/HO‐1 signaling pathway: Two birds with one stone
南方医科大学南方医院临床药学中心药剂科
Almonertinib-induced interstitial lung disease in a lung adenocarcinoma patient complicated with interstitial lung abnormality
重庆医科大学第一附属医院呼吸与危重病医学科
The Hypolipidemic Characteristics of a Methanol Extract of Fermented Green Tea and Spore of Eurotium cristatum SXHBTBU1934 in Golden Hamsters
北京工商大学轻工业学院
Sulfotransferase 1C2 promotes hepatocellular carcinoma progression by enhancing glycolysis and fatty acid metabolism
郑州大学河南医药科学研究所
Characterization of the volatile profile and its estrogenic activity in Kadsura coccinea fruit
徐州医科大学药学院
Bioassay guided isolation of caffeoylquinic acids from the leaves of Ilex pubescens Hook. et Arn. and investigation of their anti-influenza mechanism
香港中文大学生命科学学院
Berberine ameliorates depression-like behaviors in mice via inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing neuroplasticity disruption
北京大学临床研究所
Cost-effective duplex Kompetitive Allele Specific PCR markers for homologous genes facilitating wheat breeding
江苏省农业科学院小麦病害联合防治中心
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